Determination of Antagonistic Effect of CuO NPs against Bacterial Cultures
Abstract
Bacterial cultures are capable of causing infections to the humans. These bacteria can be killed or inhibited by special compounds called antibacterial agents. These compounds are generally not toxic to humans as most of the compounds are obtained from natural sources, such as, b-lactams (like penicillins), cephalosporins. Overuse of traditional antibacterial drugs, resistance may develop by bacterial cells, which ultimately leads to pose greatest health challenges by occurrence of infectious diseases. Therefore, development of non-resistance alternative antibacterial agents for better antibacterial efficacy is mandatory. This paper reports on the synthesis of copper oxide nanoparticles carried out by chemical precipitation method and explored its antibacterial efficacy against hospital borne bacterial infections. Copper oxide nanoparticles were synthesized by solvothermal route. The screening of antimicrobial activity of copper oxide nanoparticles was studied on the bacteria Staphylococcus aureus NCIM 2079 and Bacillus cereus NCIM 5293 by Anti Well Diffusion Assay (AWDA) on nutrient agar (NA) medium. It was evident that the Staphylococcus aureus was more sensitive for CuO NPs compared to Bacillus cereus. The synthesized CuO-NPs showed remarkable antibacterial activity against Bacillus cereus and Staphylococcus aureus. Minimum Inhibitory (MIC) and Minimum Bactericidal Concentration (MBC) of CuO-NPs were determined by calculating concentration dependent colony forming units per milliliter (CFU/mL) on agar plate. To synthesis of copper oxide nanoparticles carried out by chemical precipitation method and explored its antibacterial efficacy against hospital borne bacterial infections.
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References
[3] Franci G et al (2015) Silver Nanoparticles as Potential Antibacterial Agents. Molecules 20: 8856-8874. [4] Hsueh, Y. H., Tsai, P. H., & Lin, K. S. (2017). pH-dependent antimicrobial properties of copper oxide nanoparticles in Staphylococcus aureus. International journal of molecular sciences, 18(4), 793. [5] Jain, N., Bhosale, P., Tale, V., Henry, R., & Pawar, J. (2019). Hydrothermal assisted biological synthesis of Silver nanoparticles by using honey and gomutra (Cow Urine) for qualitative determination of its antibacterial efficacy against Pseudomonas sp. isolated from contact lenses. EurAsian Journal of BioSciences, 13(1), 27-33.
[6] Jin, T. and He, Y., 2011. Antibacterial activities of magnesium oxide (MgO) nanoparticles against foodborne pathogens. Journal of Nanoparticle Research, 13(12), pp.6877-6885. [7] Khan, S. A., Shahid, S., Sajid, M. R., Noreen, F., & Kanwal, S. (2017). Biogenic synthesis of CuO nanoparticles and their biomedical applications: a current review. Int J Adv Res, 5(6), 925-946. [8] Pawar, J., Bipinraj, N. K., Singh, E. A., & Nikam, N. (2012). Determination and Partial Characterization of Antimicrobial Material of Pseudomonas aeruginosa Isolated from Milk. preservation, 10, 13. [9] Pawar, J., Henry, R., Viswanathan, P., Patwardhan, A., & Singh, E. A. (2019). Testing of antibacterial efficacy of CuO nanoparticles by methylene blue reduction test against Bacillus cereus responsible for food spoilage and poisoning. Indian Chemical Engineer, 61(3), 248-253. [10] Pawar, J., Prakash, V., Vinchurkar, P., Shinde, M., Henry, R., Mandke, Y., ... & Singh, E. A. (2017). Application of Semi-Solvo Thermally Synthesized Zinc Oxide (ZnO) Nanoparticles in Food Technology and Its Characterization. Int. J. Nanotechnol. Appl, 11(1), 75-80. [11] Pawar, J., Shinde, M., Chaudhari, R., & Singh, E. A. (2017). Semi-solvo thermal synthesis and characterization of zinc oxide nanostructures against food borne pathogens. Int J Pharm Bio Sci, 8(2), 311-315. [12] Prakash, V., Pawar, J., Vinchurkar, P., Henry, R., & Patwardhan, A. (2017, March). Chemi-thermal method of synthesis of ZnO nanomaterial and its characterization. In Int. Conf. Innov. Sust. Dev. Sci. Manag. Technol (Vol. 1, p. 36). [13] Rubilar, O., Rai, M., Tortella, G., Diez, M. C., Seabra, A. B., & Durán, N. (2013). Biogenic nanoparticles: copper, copper oxides, copper sulphides, complex copper nanostructures and their applications. Biotechnology letters, 35(9), 1365-1375.
[14] Shirsat, S., Pawar, D.N., Jain, N., Pawar, J., Tale, V.S., & Henry, R. (2019). Synthesis of copper oxide nanoparticles by chemical precipitation method for the determination of antibacterial efficacy against Streptococcus sp. and Staphylococcus sp. Asian Journal of Pharmaceutical and Clinical Research, 135-138. [15] Shwe, L. T., & Win, P. P. (2013, October). Preparation of CuO nanoparticles by precipitation method. In international workshop on nanotechnology, Serpong, Indonesia (pp. 2-5).
[16] Stoimenov PK, Klinger RL, Marchin GL & Klabunde KJ (2002) Metal oxide nanoparticles as bactericidal agents. Langmuir 18: 6679-6686. [17] Subedi, S. K. (2014). An introduction to nanotechnology and its implications. Himalayan Physics, 5, 78-81. [18] Tran, T. H., & Nguyen, V. T. (2014). Copper oxide nanomaterials prepared by solution methods, some properties, and potential applications: a brief review. International scholarly research notices, 2014.
[19] Varier, K. M., Gudeppu, M., Chinnasamy, A., Thangarajan, S., Balasubramanian, J., Li, Y., & Gajendran, B. (2019). Nanoparticles: antimicrobial applications and its prospects. In Advanced Nanostructured Materials for Environmental Remediation (pp. 321-355). Springer, Cham. [20] Von Nussbaum, F., Brands, M., Hinzen, B., Weigand, S., & Häbich, D. (2006). Antibacterial natural products in medicinal chemistry—exodus or revival?. Angewandte Chemie International Edition, 45(31), 5072-5129. [21] Qureshi, N., Lee, S., Chaudhari, R., Mane, P., Pawar, J., Chaudhari, B., ... & Amalnerkar, D. (2021). Hydrothermal Generation of 3-Dimensional WO3 Nanocubes, Nanobars and Nanobricks, Their Antimicrobial and Anticancer Properties. Journal of nanoscience and nanotechnology, 21(10), 5337-5343.
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